Figure 4.

Co-expression in E. coli and purification of GST-RhpA and His-tagged Hp-RNase J. GST-RhpA (filled triangle) and His-tagged full-length or truncated Hp-RNase J (empty triangle) were co-expressed in E. coli and purified on (A) glutathione column and (B) Ni²⁺-NTA column. Panel 1 (A and B)—strain UPH574 expressing GST-RhpA + His-Hp-RNase J (RNJ); panel 2 (A and B) —strain UPH598 expressing GST-RhpA + His-ΔN-Hp-RNase J; panel A3—strain UPH600 expressing His-Hp-RNase J alone, panel B3—strain UPH597 expressing GST-RhpA alone; panel A4—strain UPH780 expressing GST and His-Hp-RNase J. The single asterisk and double asterisk denote DnaK and GroEL, respectively. Strains are listed in Supplementary Table S1. Mass spectrometry data are presented in Supplementary Table S6. CL, cleared lysate; P, purified protein.