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. 2012 Oct 22;41(1):288–301. doi: 10.1093/nar/gks945

Figure 6.

Figure 6.

Co-localization of RhpA and Hp-RNase J to the ribosomes. (A) Upper panel: polysome profile for H. pylori B128 cell extracts on a 10–50% sucrose gradient. Peaks corresponding to 30S, 50S subunits and 70S ribosomes are indicated. Numbers correspond to the collected fractions. Lower panel: western blot revealed with RhpA- and subsequently with Hp-RNase J-specific antibodies showing the distribution of RhpA and Hp-RNase J in the ribosomal fractions. Empty triangle: Hp-RNase J, filled triangle: RhpA. (B) Upper panel: polysome profile of H. pylori B128 cell extract on 10–40% sucrose gradient in the presence of 1 mM MgCl2 and 20 mM EDTA. Peaks corresponding to the dissociated 30S and 50S subunits are indicated. Lower panel: western blots showing the distribution of RhpA (filled triangle) and Hp-RNase J (empty triangle) in the collected fractions. (C) polysome profiles (10–50% sucrose gradient) and distribution of Hp-RNase J in the ΔrhpA mutant (strain UPH740). (D) Polysome profiles (10–40% sucrose gradient) and distribution of Hp-RNase J and of RhpA in the strain depleted of Hp-RNase J (UPH738, no IPTG).