Table 1.

ATPase activity of RhpA in the presence or absence of Hp-RNase J

Proteins	RNA, μg/ml
	0	100
RhpA	0.47 ± 0.27*	0.28 ± 0.28
RhpA + BSA	nd	0.35 ± 0.33
RhpA + RNase J	0.47 ± 0.34	2.81 ± 0.15
RhpA + ΔN-RNaseJ	0.90 ± 0.11	2.44 ± 0.81
RNaseJ	nd	0.57 ± 0.55
ΔN-RNaseJ	nd	0.20 ± 0.20
Ded1	2.52 ± 0.14	18.12 ± 0.66

ATPase activity is presented in moles of ATP hydrolysed per minute per mole of the enzyme. Protein mixtures were at equimolar amounts. Total RNA from yeast (Ambion) was used at 100 µg/ml. Ded1 helicase from yeast was used as a reference. Mean values and standard deviations were calculated from at least two independent experiments. nd, non-determined.