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. 2012 Nov 7;41(1):533–541. doi: 10.1093/nar/gks1013

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 3. — The 5.8S rRNA processing defects of mtr4-archless and rrp6Δ are not additive. RNA was isolated from each of the strains indicated and analyzed by northern blotting of a urea polyacrylamide gel. Two rrp6Δ strains are included; lane 3 is an rrp6Δ MTR4 strain, whereas lane 4 is an rrp6Δ mtr4Δ strain complemented with an MTR4 plasmid for direct comparison with lane 5. Northern blot was performed using the probes complementary to 5.8S rRNA and SRP for loading control.