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. 2013 Mar 8;5(1):e00107. doi: 10.1042/AN20120075

Figure 2. Sox2 and Olig2 expression in the SVZ and in NS cultures.

Figure 2

For all images, Sox2 is labelled in green and Olig2 is labelled in red. (A–D) Sox2 and Olig2 immunohistochemical analysis in coronal brain sections belonging to neonatal rat brains. (E–H) Sox2 and Olig2 expression analysis in the SVZ wall of coronal brain sections belonging to neonatal rat brains. (I–L) Sox2 and Olig2 expression analysis in non-dissociated NS. (K) A cell that co-expresses both Sox2 and Olig2 is indicated with an arrow, while a Sox2+/Olig2− cell is indicated with a white arrowhead and a Sox2−/Olig2+ cell is indicated with an empty arrowhead. (M–P) Quantitative analysis of the cell proportions expressing either Sox2 or Olig2. (O) Nuclei expressing both Olig2 and Sox2 are indicated with empty arrowheads, while Sox2+/Olig2− nuclei are indicated with white arrowheads and a Sox2−/Olig2+ nucleus is indicated with an arrow. Bars in (P) represent means±S.E.M. for each cell population. The scale bar (A) represents 250 μm for (A)–(D), scale bar in (E) represents 10 μm for (E)–(H), and scale bar in (I) represents 100 μm for (I)–(O).