Table 1. Primary antibodies used for ICC and WB.

Antigen (Clone)	Immunogen	Species	Isotype, clonality*	Brand	Dilution for ICC (WB)
Acetylated α-tubulin (6-11B-1)	Outer arm of Strongylocentrotus purpuratus	Mouse	IgG2b, monoclonal	Sigma–Aldrich®	1:1000
Activated caspase III	Peptide RGTELDCGIETD of human activated caspase III with added KLH amino acids	Rabbit	Monoclonal†	Cell Signaling Technologies	1:100
BrdU (BMC 9318)	BrdU conjugated to BSA	Mouse	IgG1, monoclonal	Roche	1:100
GFAP	Amino acids 1–428 of GFAP from bovine spinal cord	Chicken	IgY, polyclonal	Neuromics	1:500
GFAP-Cy3 (G-A-5)	GFAP from pig spinal cord	Mouse	IgG1, monoclonal	Sigma–Aldrich®	1:1000
Human Tf	Human seric Tf synthetic peptide	Chicken	IgY, polyclonal	GeneTex	1:100
MBP	Amino acids 1 to 154 from rat MBP N-terminal fragment	Rabbit	Polyclonal	Dr A. Campagnoni Lab (UCLA, USA)	1:500
Mouse Tf (I-20)	N-terminal peptide of mouse Tf	Mouse	Polyclonal	Santa Cruz Biotechnologies	1:50
Nestin	Peptide containing peptide sequence shared between human and rat Nestin	Chicken	Polyclonal	Neuromics	1:100
NG2	NG2 proteoglycan chondrotin sulfate purified from rat	Rabbit	IgG, polyclonal	Millipore	1:200
Olig2 (211F1.1)	Human recombinant Olig2	Mouse	IgG2a, k, monoclonal	Millipore	1:100
PDGFRα	Mouse recombinant PDGFRα	Goat	Polyclonal	Neuromics	1:100
Rat Tf	Unknown	Rabbit	Polyclonal	Dr. Zakin laboratory (Institute Pasteur, France)	1:100 (1:2500)
Sox2	Synthetic peptide sequence containing amino acids 300 to C-terminal end of human Sox2 with added KLH amino acids	Rabbit	IgG, Polyclonal	Abcam	1:100
TfRc1 or CD71 (42/6)	Human purified TfRc1	Mouse	IgA, monoclonal	Millipore	1:20
TfRc1 or CD71 (OX-26)	Phytohemagglutinine-activated lymph node cells	Mouse	IgG2a,k, monoclonal	BD Pharmingen™	1:50

*The polyclonal antibodies with no assigned isotype indicate they contained more than one isotype or the isotype was not specified by the manufacturer.

†We did not have access to data regarding the antibody isotype.