Table 2. PCR primer sequences.

Gene	NCBI GenBank® ID	Protein	Sequence (5′→3′)*	Primer	Exon	Brand
Gapdh	NM_017008.4	Glyceraldehyde-3-phosphate dehydrogenase	TTAGCCCCCCTGGCCAAGG	Forward	5	IDT®
			CTTACTCCTTGGAGGCCATG	Reverse	8	IDT®
Trf	NM_001013110.1	Transferrin	TTCTCATGCTGTTGTGGCTC	Forward	7	IDT®
			CAGGTCACAGAGGGTGGAAT	Reverse	13	IDT®
Tfrc1	NM_022712.1	Transferrin receptor type 1	GCGAAGTCCAGTGTGGGAACAGGT	Forward	10	IDT®
			GTAGCACGGAAGTAGTCTCCACGAG	Reverse	17	IDT®
Tfrc2	NM_001105916.1	Transferrin receptor type 2 (α and β)*	GCTTAGGCTCCTGTCCTGG	Forward	4	Sigma–Aldrich®
			GTAGCTGCTCTTGGACGCTC	Reverse	7	Sigma–Aldrich®

*The TfRc2 primers were designed to obtain a single 483 bp band for both α and β transcripts of the gene.