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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: Virology. 2012 Feb 2;425(2):133–142. doi: 10.1016/j.virol.2012.01.009

Figure 6. RhCMV miR-Rh183-1 is homologous to HCMV miR-US5-2.

Figure 6

(A) Sequence alignment of miR-Rh183-1 and miR-US5-2. The seed sequence of each miRNA is underlined. (B) 293 cells were co-transfected with dual luciferase reporters encoding the WT Rh186 and US7 3’UTRs or reporters with mutations in the miRNA target sites (Rh186mut and US7mut) as well as miRNA mimics of miR-Rh183-1, miR-US5-2 or a negative control miRNA. 24 hours after transfection, cells were harvested and luciferase activity was monitored. Experiments were performed in triplicate and presented as mean +/- standard deviation with the negative control set to 1.