. Author manuscript; available in PMC: 2013 Apr 10.

Published in final edited form as: Virology. 2012 Feb 2;425(2):133–142. doi: 10.1016/j.virol.2012.01.009

Table 1.

miRNA RT-PCR primer and probe sets

Target	RT primer^a	Taqman Forward Primer	Probe
miR-Rh04	TCACCA	GCGGTCCTTTGTATA	TGGATACGACTCACCA
miR-Rh16	CTAGAG	GCGCTGGGACCGAGTAGCT	TGGATACGACCTAGAG
miR-Rh70	CACTGC	GCGGGACGCATCTGGGAGCT	TGGATACGACCACTGC
miR-Rh94	CACGGC	GCGCTGGCGACCTGGGCAT	TGGATACGACCACGGC
miR-Rh96-1	CGCCTG	GCGGGCAGTGCTGGATCGGG	TGGATACGACCGCCTG
miR-Rh96-2	TGGCAT	GCGGAAGGCCGGCTTCTCGG	TGGATACGACTGGCAT
miR-Rh96-3	AAGAGG	GCGGCGGGCCAACCTAGC	TGGATACGACAAGAGG
miR-Rh106-1	ACGACC	GCGGAACTCTCGGATGAGGT	TGGATACGACACGACC
miR-Rh106-2	ATGCTG	GCGGACTTGTGGTTTCTGG	TGGATACGACATGCTG
miR-Rh156-1	CATGAG	GCGGATATGGCGTCTATGG	TGGATACGACCATGAG
miR-Rh156-2	GGCCAC	GCGGCATTGACGTCAATGG	TGGATACGACGGCCAC
miR-Rh157	AGTCCA	GCGGTTCAATATGGCGTTT	TGGATACGACAGTCCA
miR-Rh159	ACAACC	GCGGTGCCTTATGGTCGTT	TGGATACGACACAACC
miR-Rh176	ACGCGG	GCGGTAGGCCCTCTCTACC	TGGATACGACACGCGG
miR-Rh183-1	GATTTC	GCGCTTTATGATCGGTGCGA	TGGATACGACGATTTC
miR-Rh183-2	CACACC	GCGGGACGGTCAACGGTA	TGGATACGACCACACC
miR-Rh225	TCTCCA	GCGGTCCGGAACGGGAGAC	TGGATACGACTCTCCA

All RT primers share the sequence GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC, with the last six unique 3’ nucleotides listed in the table