Table 2.

Expression levels of genes of the biphenyl pathway, bphA, bphB, bphC, and bphD, in B. xenovorans LB400 after 18 hours of cultivation. Left Panel: Cells growing on biphenyl (BP) (770 mg L⁻¹, 5 mM) relative to cells growing on succinate (1,180 mg L⁻¹, 10 mM). Right Panel: Cells growing on succinate (1,180 mg L⁻¹, 10 mM) exposed to 5 mg L⁻¹ of BP, 4-chlorobiphenyl (CB), 2′-hydroxy- (OH-), 3′-OH-, and 4′-OH-4-CB relative to non-exposed cells

Biphenyl		Succinate
Genes	BP (770 mg L−1)	BP (5 mg L−1)	4-CB (5 mg L−1)	2′-OH-4-CB (5 mg L−1)	3′-OH-4-CB (5 mg L−1)	4′-OH-4-CB (5 mg L−1)
bphA	6.5 (5.9-7.1)	2.5 (2.4-2.6)	2.0 (1.9-2.2)	1.6 (1.4-1.7)	0.25 (0.23-0.28)	0.29 (0.28-0.30)
bphB	6.7 (5.1-8.7)	3.1 (2.9-3.2)	2.2 (1.9-2.6)	2.2 (1.9-2.5)	0.66 (0.51-0.85)	0.46 (0.37-0.58)
bphC	7.6 (6.9-8.3)	3.8 (3.6-3.9)	2.8 (2.4.-3.2)	2.4 (1.8-3.2)	0.46 (0.42-0.49)	0.41 (0.33-0.50)
bphD	8.8 (8.0-9.6)	2.9 (1.8-4.7)	2.3 (1.6-3.2)	2.2 (1.8-2.5)	0.21 (0.19-0.24)	0.16 (0.14-0.17)

Results are presented as mean of three replicates, the ranges of amplification levels were calculated based on the standard deviations of ΔΔC_T values