Skip to main content
. 2013 Mar 8;8(3):e58662. doi: 10.1371/journal.pone.0058662

Figure 4. Spermine, a mitochondrial uniporter agonist, and p38 inhibitor SB202190 suppress glucose deprivation-induced IL-6 gene expression.

Figure 4

Real-time RT-PCR of IL-6 was carried out with RNA extracted from BV2 microglia incubated in a normoxic condition with or without 1.5 mM spermine (A) or 10 µM SB202190 (B). **p<0.01 vs glucose deprivation without spermine or SB202190. (C) Cell lysates from cells incubated in a normoxic condition were separated on SDS-polyacrylamide gels and analyzed with antibodies against P-p38, p38, P-JNK, JNK, eIF2α, or P-eIF2α. Quantitation of protein levels of P-p38, P-JNK, P-eIF2α was calculated with those of non-phosphorylated p38, JNK, eIF2α respectively. Ratios of protein levels of interest to those of non-phosphorylated forms obtained from cells incubated in the glucose free medium without spermine for 0.5 h were calculated as 1 in each set of experiments for statistical analysis. Results are shown as means ± SD; n = 3 independent experiments. *p<0.05 vs glucose free medium.