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. 2013 Mar 8;8(3):e58662. doi: 10.1371/journal.pone.0058662

Figure 6. GSK-3β inhibitor enhances glucose deprivation-induced IL-6 gene expression.

Figure 6

Real-time RT-PCR of IL-6 (A) was carried out with RNA extracted from BV2 microglia incubated in a normoxic condition with or without GSK-3 inhibitor CHIR99021. (B) Whole cell lysates from cells incubated in a normoxic condition were separated on SDS-polyacrylamide gels and analyzed with antibodies against β-tubulin, IκB, P-p38, p38, P-JNK, or JNK. (C) Quantitation of protein levels of IκB, P-p38, and P-JNK was carried out as described in Fig. 4C.