Figure 7. Akt inhibitor reverses effects of hypoxia on IL-6 and iNOS gene expression in BV2 microglia.

(A) Cells in serum-free medium were incubated for periods indicated in one of the following conditions; media containing glucose in an incubator with 21% oxygen, media containing glucose in a hypoxia chamber with 5% oxygen, media without glucose in an incubator with 21% oxygen, or media without glucose in a hypoxia chamber with 5% oxygen. Whole cell lysates separated on SDS-polyacrylamide gels were analyzed with Akt or P-Akt. (B) Real-time RT-PCR of IL-6 was carried out with RNA extracted from BV2 cells incubated with or without Akt inhibitor. (C) Real-time RT-PCR of IL-6 was carried out with RNA extracted from BV2 cells incubated with or without 10 µM FM19G11, an inhibitor of HIF-1/2α.