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. 2013 Mar 8;8(3):e58480. doi: 10.1371/journal.pone.0058480

Figure 1. Detection of antigens and DNA of T. cruzi in urine of guinea pigs experimentally infected.

Figure 1

1.A. Antigenic bands in urine samples of guinea pigs infected with T. cruzi. Bands were detected by Western Blot using a polyclonal antibody against excretory-secretory trypomastigote T. cruzi antigen (TESA). C-: Negative control (RPMI 1640 medium). C+: Positive control (TESA antigen). MW: molecular weight marker. Urine samples of infected guinea pigs: Lane 1) 165 dpi, lane 2) 25 dpi, lane 4) 115 dpi, and lane 5 and 7) 55 dpi. Urine samples of non- infected guinea pigs: Lanes 3, 6 and 8. Bands under 70 kDa were considered unspecific because 25% of the non-infected guinea pigs had a reaction to these low bands. 1. B. Detection of trans-renal DNA in urine samples of guinea pig infected with T. cruzi. Bands were detected by PCR using primers TcZ1/TcZ2. C+: Positive control (DNA of T. cruzi from medium culture). MW: molecular weight marker. Urine samples of infected guinea pigs: Lane 1) 25 dpi, lane 3) 55 dpi, lane 4) 40 dpi, lane 5) 55 dpi, and lane 7) 25 dpi. Urine samples of non- infected guinea pigs: Lanes 2 and 6.