Table 1. Kinetics of antigen detection in urine samples of guinea pig infected with Trypanosoma cruzi.
| Bands | 75 kDa | 80 kDa | 120 kDa | 150 kDa | |||||
| Days pi | N | n | % | n | % | n | % | n | % |
| 15 | 8 | 0 | 0.0 | 0 | 0.0 | 0 | 0.0 | 0 | 0.0 |
| 20 | 8 | 7 | 75.0 | 7 | 100.0 | 0 | 0.0 | 0 | 0.0 |
| 25 | 8 | 8 | 100.0 | 8 | 100.0 | 3 | 37.5 | 3 | 37.5 |
| 40 | 8 | 6 | 100.0 | 6 | 100.0 | 2 | 25.0 | 2 | 25.0 |
| 55 | 8 | 6 | 75.0 | 6 | 75.0 | 3 | 37.5 | 3 | 37.5 |
| TOTAL | 40 | 27 | 67.5 | 27 | 67.5 | 8 | 20.0 | 8 | 20.0 |
| 115 | 8 | 2 | 25.0 | 2 | 25.0 | 4 | 50.0 | 4 | 50.0 |
| 165 | 8 | 2 | 25.0 | 2 | 25.0 | 4 | 50.0 | 4 | 50.0 |
| 365 | 4 | 1 | 33.3 | 1 | 33.3 | 1 | 33.3 | 1 | 33.3 |
| TOTAL | 20 | 5 | 25.0 | 5 | 25.0 | 9 | 45.0 | 9 | 45.0 |
Bands were detected by Western Blot using a polyclonal antibody against excretory-secretory trypomastigote T. cruzi antigen.
N: Number of animals per group.
n: Number of animals with antigenuria test positive.
%: Percentage of positive animals.
The antigenuria test was considered positive when any of the bands of 75, 80, 120 or 150 kDa were detected.