Figure 2. RTEX+TIG cells have undetectable levels and activity of mitochondrial respiratory chain complexes.

Total proteins were extracted from untreated TEX and RTEX+TIG cells, as well as TEX cells treated with 2.5 µM and 5 µM tigecycline for 24 hours. A Levels of Cox-1, Cox-2, Cox-4 and tubulin were measured by immunoblotting. B Complex II, III and IV enzyme activity relative to citrate synthase activity was determined as described in the Materials and Methods section. Results shown as mean ± SD of three independent experiments.