Table 3. Mean quantification cycle (Cq) values of viral genome detection via EBLV-1 and EBLV-2 specific real-time RT-PCR.
| Participants | |||||||||||||||||||
| Species | Freuling unpublished | Wakeley et al., 2005 | in-house assay | ||||||||||||||||
| A3 | A4 | O1 | O2 | E1* | E2* | F3 | F4 | I1 | I2 | J1 | L1 | L2 | M1 | M2 | N3 ts | N4 ts | P3 | P4 | |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 31# | – | – | – |
| RABV (I) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| neg | – | – | ? (32) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 24# | - | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | ? (36) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| EBLV–1 | 18 | – | 21 | – | 20 | – | 26 | – | 28 | – | 28 | 24 | – | 26 | – | 31 | – | 20 | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 38# | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| EBLV–2 | – | 17 | ? (13) | 21 | – | 20 | – | 22 | – | 28 | 18 | – | 25 | – | 24 | – | 29 | – | 17 |
| neg | – | – | ? (29) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV (II) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV (III) | – | – | ? (25) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| RABV (0) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
RABV: Rabies virus;;EBLV: European Bat Lyssavirus;neg.: negative control; –: not applicable; #: cross–reactivitiy with other Lyssavirus species; ?: inconclusive based on curve shape; dilution series (0), (I), (II), (III); 100, 10−1, 10−2, 10−3;
*
Hoffmann and Müller personal communication; ts: two-step systems; no duplicates for assays M1 and M2; all laboratories except J1 used separate species-specific real-time PCRs to detect EBLV-1 or -2.