Figure 2. Suppression of Txnip expression by proinflammatory stimulation.

BMDM (A), NIH3T3 (B), or RAW264.7 (C) cells were stimulated with 2 µM CpG DNA 100 ng/ml LPS, 10 ng/ml IL-1β, 10 ng/ml TNF-α, or 10 ng/ml IL-10. Cells were lysed at the indicated time after stimulation. Txnip, β-actin, phosphorylated STAT3 (pSTAT3), or STAT3 proteins were detected by western blotting. Data shown are a representative of at least three independent experiments.