Figure 3. Essential roles for ChoREs and CCAAT boxes in the Txnip promoter.

(A and B) Luciferase reporter plasmids containing indicated Txnip promoter fragments with or without point mutations (mt) at ChoREs, CCAAT boxes, or a FOXO binding site were transfected to RAW264.7 cells together with phRL-TK-luc. Transfected cells were stimulated with or without 100 ng/ml LPS for 4 h, then lysed for luciferase activity measurement. Relative luciferase activities normalized by internal control are shown as mean ± S.E. of triplicate samples. Data shown are a representative of at least three independent experiments.