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. 2013 Mar 8;8(3):e58935. doi: 10.1371/journal.pone.0058935

Figure 4. Schlafen-4 is a Gli1-target gene that marks the CD11b+CD11c+ gastric myeloid population.

Figure 4

A) Microarray heat map of sham- and H. felis-infected WT and Gli1−/− stomachs. Each column represents pooled stomach RNA samples from two mice. B) RT-qPCR analysis of Slfn-4 mRNA from sham- or 6 month H. felis-infected WT, Gli1+/−, or Gli1−/− stomachs. C) ChIP analysis of two Slfn-4 promoter sequences in mouse Gli1 promoter in RAW264.7 cells transfected with Gli1-FLAG-overexpressing or the empty vector plasmid. D) Immunofluorescence analysis of CD11b (green), CD11c (magenta), Slfn-4 (red) in 6-month H. felis infected WT mice. Error bars represent the mean +/− SEM. N = 5–10 mice per group. **p<0.01; *p<0.05.