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. 2013 Mar 8;8(3):e57035. doi: 10.1371/journal.pone.0057035

Figure 2. Suppression of amber, opal, and ochre mutations at N134 of sfGFP by their corresponding PylRS-tRNAPyl pairs in the absence and presence of BocK.

Figure 2

(A) Proteins shown in the gel represent their real relative sfGFP expression levels. Lanes 1 and 2 were transformed with pETtrio-PylT(CUA)-PylRS-sfGFP134TAG; lanes 3 and 4 were transformed with pETtrio-PylT(UUA)-PylRS-sfGFP134TAA; lanes 5 and 6 were transformed with pETtrio-PylT(UCA)-PylRS-sfGFP134TGA. ESI-MS spectra of sfGFP expressed in cells (B1) transformed with pETtrio-PylT(CUA)-PylRS-sfGFP134TAG and grown in the presence of 5 mM BocK, (B2) transformed with pETtrio-PylT(UUA)-PylRS-sfGFP134TAA and grown in the presence of 5 mM BocK, (B3) transformed with pETtrio-PylT(UCA)-PylRS-sfGFP134TGA and grown in the absence of BocK, and (B4) transformed with pETtrio-PylT(UCA)-PylRS-sfGFP134TGA and grown in the presence of 5 mM BocK.