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. 2013 Mar 8;8(3):e57035. doi: 10.1371/journal.pone.0057035

Figure 3. Suppression of an opal mutation at S2 of sfGFP by the PylRS-Inline graphic pair.

Figure 3

(A) Expression of sfGFP with an opal mutation. Lanes 1 and 2 were transformed with pETtrio-pylT(UCA)-sfGFP134TGA and grown in the absence or presence of 5 mM BocK; lanes 3 and 4 were transformed with pETtrio-pylT(UCA)-sfGFP2TGA and grown in the absence or presence of 5 mM BocK. Each protein shown in the gel represents their real relative expression levels. ESI-MS of sfGFP expressed in cells transformed with pETtrio-pylT(UCA)-sfGFP2TGA and grown in the (B1) absence or (B2) presence of 5 mM BocK.