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. 2013 Mar 8;8(3):e57035. doi: 10.1371/journal.pone.0057035

Figure 5. Suppression of an opal mutation at N134 of sfGFP by different Inline graphic variants.

Figure 5

(A) Proteins shown in the gel represent their real relative expression levels. Lanes 1 and 2 were transformed with pETtrio-pylT(UCA)G73C-sfGFP134TGA and grown in the absence or presence of 5 mM BocK; lanes 3 and 4 were transformed with pETtrio-pylT(UCA)G73A-sfGFP134TGA and grown in the absence or presence of 5 mM BocK; lanes 5 and 6 were transformed with pETtrio-pylT(UCA)G73U-sfGFP134TGA and grown in the absence or presence of 5 mM BocK; lanes 7 and 8 were transformed with pETtrio-pylT(UCA)-sfGFP134TGA and grown in the absence or presence of 5 mM BocK. The ESI-MS analysis of sfGFP expressed in cells transformed with pETtrio-pylT(UCA)G73U-sfGFP134TGA and grown in the (B1) absence or (B2) presence of 5 mM BocK.