Figure 6. Cross recognitions between different anticodons of tRNA^Pyl and nonsense mutations at N134 of sfGFP.

Cells were transformed with pETtrio-PylT(NNN)-PylRS-sfGFP134N’N’N’ and grown in the presence of 5 mM BocK (NNN and N’N’N’ denote anticodons and codons specified in the figure). Proteins shown in the gel represent their real relative expression levels.