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. 2013 Mar 8;8(3):e58139. doi: 10.1371/journal.pone.0058139

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© 2013 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Recombinant FADS proteins were digested with PP and visualized by Coomassie blue staining. Triangles indicate the recombinant desaturases before and after PP digestion. Arrow indicates Precision protease proteins. M: protein marker. (B) Diagram of recombinant desaturase structures with indicated molecular weight and cleavage sites. Long arrow indicates the Precision protease digestion site. Short arrow indicates the cleavage site before the Cyto b5 domain.