Table 1. Purification of M. alpina desaturases.
Step | FADS15 protein | FADS12 protein | FADS9-I protein | |||
Conc. (mg/L) | Yield (%) | Conc. (mg/L) | Yield (%) | Conc. (mg/L) | Yield (%) | |
Cell lysate | 130.0 | 100.0 | 110.0 | 100.0 | 350.0 | 100.0 |
Centrifugation (500–10,000 g) | 112.0 | 86.2 | 82.0 | 72.7 | 254.0 | 72.6 |
Detergent extract | 76.0 | 58.5 | 74.8 | 68.2 | 223.0 | 63.7 |
Ni-NTA 20 mM ID(5 mM ID) | 4.6(5.5) | 3.5(4.2) | 2.5(4.0) | 2.3(3.6) | 37.5(92.4) | 10.7(26.4) |
ID: imidazole.