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. 2013 Mar 8;8(3):e58139. doi: 10.1371/journal.pone.0058139

Table 1. Purification of M. alpina desaturases.

Step FADS15 protein FADS12 protein FADS9-I protein
Conc. (mg/L) Yield (%) Conc. (mg/L) Yield (%) Conc. (mg/L) Yield (%)
Cell lysate 130.0 100.0 110.0 100.0 350.0 100.0
Centrifugation (500–10,000 g) 112.0 86.2 82.0 72.7 254.0 72.6
Detergent extract 76.0 58.5 74.8 68.2 223.0 63.7
Ni-NTA 20 mM ID(5 mM ID) 4.6(5.5) 3.5(4.2) 2.5(4.0) 2.3(3.6) 37.5(92.4) 10.7(26.4)

ID: imidazole.