Figure 6. Pro-apoptotic effects of VPA or SAHA on Kasumi-1 cells. (A) Kasumi-1 cells were incubated or not (time 0) in the presence of 2 mM VPA or 1 μM SAHA for the indicated times (hours). Cells were then lysed and western blotting performed with the indicated antibodies. Results are from one typical experiment out of three. (B) Cells were incubated without (NT) or with 2 mM VPA or 1 μM SAHA for the indicated times (days) and apoptosis was measured by the Annexin V test and flow cytometry. Values are means ± SEM of the percentages of apoptotic cells obtained from three independent experiments. The statistical significance of differences was determined by the Student’s t-test for paired samples (*p < 0.05; **p < 0.01). (C) Blasts from two t(8;21) AML patients (#1 and #2) were incubated or not (NT) in the presence of 2 mM VPA or 1 μM SAHA for the indicated times (hours). Cells were then lysed and western blotting performed with the indicated antibodies.