Figure 1. Methylation patterns of selected miRNAs in the cell line panel. Methylation as determined in human foreskin keratinocytes (HFKs), HPV-transformed keratinocyte cell lines FK16A, FK18A and FK18B (reminiscent of high-grade CIN), and cervical cancer cell lines SiHa, HeLa and CaSki is shown for (A) hsa-miR-149, (B) hsa-miR-203, (C) hsa-miR-375, (D) hsa-miR-572 and (E) hsa-miR-638. In (F) ACTB results are shown, indicating successful modification and comparable input for all samples. In vitro methylated DNA (IVD) and unmodified DNA (UD) were included as a positive and negative control, respectively.