Table 1. Primer and probe sequences used for (q)MSP analysis.

Gene	Primer and probe sequences (5′−3′)	Amplicon (bp)	Annealing (°C)
hsa-miR-149	F: GTCGTGCGTTTAGTTTGTAGCG R: CGACCTAAAAACCCCCGAC P: CAAAACGAACGAAAACCTAAAAACCTCGAACA	111	59 58 68
hsa-miR-203	F: GTCGGTTTTCGGGATTCGTA R: CGATTCGCCGAACTACGAAA P: AACCGCCCGCGTC-Xsprobe	78	59 60 70
hsa-miR-210	F: GTCGGGTTGGGTAGGCG R: CACTACCGAATAAACGAACGAAAAAA	90	59 61
hsa-miR-375	F: GGGGCGTTGTGTAGTATTGAGTTC R: GAAACGAAAACGAAAAACCCG P: AAACCTAACGATCGCCCTCGATAATCTCCTAATC	91	60 60 70
hsa-miR-572	F: TCGGTTTATTTTTTAGTTTCGTAGTGC R: CACGACCTCGACACCTCCTAA	111	59 59
hsa-miR-638	F: TCGGTTAGAGTTGTTATTTGGATTTG R: CCGACCCACGAAATTCTCC	96	59 59
ACTB	F: TGGTGATGGAGGAGGTTTAGTAAGT R: AACCAATAAAACCTACTCCTCCCTTAA P: ACCACCACCCAACACACAATAACAAACACA	133	58 59 69

F, forward; R, reverse; P, probe; Xsprobe, minor groove binder probe. Methylated CpG dinucleotides are in bold. Changed positions in the sequence due to the modification are indicated in italic.