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Effect of opioid peptide treatment of the developmental pattern of dre-oprm1. dre-oprm1 expression was assayed by in situ hybridization on whole-mount embryos that were exposed to 10 μmol/L Met-ENK, MEGY or β-END from 5 to 24 hpf or 48 hpf. Embryos are oriented anterior toward the left and posterior toward the right. A total of 25 embryos were used for each treatment and for each time point, and the experiment was repeated three times.
