Table 5.

Changes in the expression of zebrafish proenkephalins and proopiomelanocortin in dre-oprm1 morphants.

	dre-penk a	dre-penk b	dre-pomc a
24 hpf	1.37 ± 0.04a	1.54 ± 0.14a	1.03 ± 0.29
48 hpf	0.54 ± 0.10a	0.98 ± 0.04	0.88 ± 0.20

Results represent the relative gene expression fold-changes of dre-penk a, dre-penk b and dre-pomc a in morpholino-injected embryos of 24 and 48 hpf. Data shown are normalized to dre-eef1a1l1. Note the differential regulation of enkephalin- and β-END–containing genes in the morphant embryos. A total of 50 embryos were used for each treatment and for each time point; the experiment was performed in triplicate and repeated four times.

^ap < 0.05 (unpaired Student t test, n = 4).