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. Author manuscript; available in PMC: 2013 Nov 6.
Published in final edited form as: Anal Chem. 2012 Oct 29;84(21):9379–9387. doi: 10.1021/ac302165h

Figure 5.

Figure 5

Photobleaching analysis of FRET signal of a single hybridized DNA spot. Comparison of Cy3 and Cy5 emission intensities in unbleached and bleached regions of a single hybridized DNA microspot. A) Images of both unbleached and bleached areas (dotted oval) of the same DNA spot after confocal local photobleaching. B) Quantification of both Cy3 probe and Cy5 target emission intensities before and after photobleaching (solid squares) showing reduction of Cy5 target signal and increase in Cy3 probe signal once Cy5 fluorophores are photolyzed, consistent with FRET effects.