Fig. 3.

Involvement of ER stress mediators, eIF2α, XBP-1 and ATF6, in the thapsigargin-triggered reduction of EC-SOD. (A) 3T3-L1 adipocytes were treated with 1 µM thapsigargin (Tg) or 1 µg/mL tunicamycin (Tu) for 1 h (A, D) or 24 h (B, C). After the cells had been treated, Western blotting (A, D) and RT-PCR (B, C) were carried out. RT-PCR data (B) were normalized using GADPH levels (**p<0.01 vs vehicle (V), ^##p<0.01 vs Tu-treated cells).