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. 2013 Mar;15(3):296–304. doi: 10.1593/neo.122044

Figure 1.

Figure 1

AXL expression enhances survival of EAC cells. (A) OE33 cells were stably transfected with AXL or pcDNA4 control plasmids and subjected to Western blot analysis of AXL protein. (B) OE33/pcDNA4 and OE33/AXL stable cells were treated with vehicle or with the indicated concentrations of recombinant TRAIL for 5 hours. Cell viability was evaluated by CellTiter-Glo Luminescent Cell Viability Assay. Cell survival of AXL-expressing cells was significantly higher than control cells in response to TRAIL. (C) OE19 cells were infected with control (10 MOI, multiplicity of infection) or AXL (10 MOI) adenoviruses and subjected to Western blot analysis of AXL protein. (D) Cell viability of OE19 cells transiently expressing control empty vector or AXL in response to TRAIL was evaluated as in B. Survival of cells expressing AXL was significantly higher than control cells in response to TRAIL. (E) FLO-1 cells were transduced with lentivirus particles expressing AXL shRNA or control shRNA and subjected to Western blot analysis of AXL protein. (F) Cell viability of FLO-1 cells stably expressing AXL shRNA or control shRNA in response to TRAIL was assessed as in B. Knockdown of endogenous AXL in FLO-1 cells significantly decreased cell survival in response to TRAIL. Results are representative of at least three experiments and shown as means ± SD. *P < .05, **P < .01.