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. 2013 Mar;15(3):296–304. doi: 10.1593/neo.122044

Figure 5.

Figure 5

AXL modulates caspase-8/FADD protein interaction in response to TRAIL. Western blot analysis of immunoprecipitated proteins with control IgG or FADD antibodies in OE33 cells stably expressing pcDNA4 or AXL (A) and FLO-1 cells stably expressing control shRNA or AXL shRNA (B), after treatment with TRAIL (40 ng/ml) for 5 hours. The data indicated that the reconstitution of AXL expression in OE33 cells had no effect on DR5/FADD interaction but attenuated the recruitment of pro-caspase-8 by FADD in response to TRAIL. Conversely, knockdown of endogenous AXL in FLO-1 cells significantly promoted the interaction between FADD and caspase-8 without affecting DR5/FADD association in response to TRAIL.