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The anti-HER3 D1 Abs 16D3-C1 and 9F7-F11 and the anti-HER3 D3 Ab H4B-121, but not the Abs 12H8-B11, H4B-25, and H3A-122, block HER2/HER3 heterodimerization in NIH/3T3 fibroblasts that express HER2 and HER3. The concentration of HER3 Abs or NRG is indicated. The blockade of HER2/HER3 heterodimerization was quantified by using Ab-based TR-FRET assays.
