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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Cell Biochem. 2013 Jan;114(1):67–78. doi: 10.1002/jcb.24301

Fig. 1.

Fig. 1

ZA treatment effects on bone area and osteoclast activity. Four-week-old C57BL/6J male mice were treated with 200 μg/kg of ZA twice/week for 4 weeks. A–D: ZA treatment increased bone area. (A–D) Histomorphometric analysis of tibiae shows an increase of bone volume (BV/TV %), trabecular thickness (Tb Th, mm) and number(Tb number mm−1), and decreased trabecular spacing (Tb Sp,) in tibiae from ZA treated mice versus vehicle (VEH); E: blood serum TRAP5b levels were reduced with ZA treatment; F: representative TRAP staining of tibia; G–H: bone marrow calcium levels were decreased and serum calcium levels were not changed; Calcium levels were measured in the bone marrow (G) and serum (H) of mice treated with ZA or vehicle for up to 4 weeks; (T/C) Treatment/control (*P <0.05; **P <0.01; ***P <0.005 versus vehicle [VEH]). Data are presented as mean ± SEM, n = 8/gp (A–E, G–H).