Effects of ZA on bone and vasculature in 16-week-old mice. ZA did not alter vessel volume (VV/TV), thickness (V Th), or number(VN) in adult mice, but still increased LSK numbers. Sixteen-week-old mice were treated with ZA or vehicle (VEH) for 4 weeks. A: Bone marrow cells were collected and LSK were analyzed by flow cytometry. B: H&E staining of mice treated with vehicle or ZA shows similar vessels in the trabecular area of tibiae. C: Representative MicroCT images of bones with microfil perfusion. D–G: MicroCT analyses of microfil for vessel volume/tissue volume (VV/TV%), thickness (V Th, mm), number (VN, mm−1), and spacing (VS, mm; **P <0.005). Data are presented as mean ± SEM, n = 8/gp.