GRSF1 Colocalizes with Nascent Mitochondrial RNA
(A) Confocal analysis of HeLa cells immunolabeled with anti-GRSF1 and anti-mtSSB. Arrowheads, foci formed by GRSF1 in mitochondria. Bottom panels, magnification.
(B) Confocal analysis of HeLa cells transfected with GRSF1-HA, labeled with BrU, and immunostained with anti-HA and anti-BrU antibodies. Arrowheads, foci formed by GRSF1 in mitochondria. Bottom panels, magnification.
(C) Quantification of (A) and (B). Data are shown ± SEM (N ≥ 5 cells with ≥20 foci per cell; in total ≥ 100 foci were analyzed).