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. 2013 Mar 5;17(3):399–410. doi: 10.1016/j.cmet.2013.02.005

Figure 2.

Figure 2

GRSF1 Colocalizes with Nascent Mitochondrial RNA

(A) Confocal analysis of HeLa cells immunolabeled with anti-GRSF1 and anti-mtSSB. Arrowheads, foci formed by GRSF1 in mitochondria. Bottom panels, magnification.

(B) Confocal analysis of HeLa cells transfected with GRSF1-HA, labeled with BrU, and immunostained with anti-HA and anti-BrU antibodies. Arrowheads, foci formed by GRSF1 in mitochondria. Bottom panels, magnification.

(C) Quantification of (A) and (B). Data are shown ± SEM (N ≥ 5 cells with ≥20 foci per cell; in total ≥ 100 foci were analyzed).