Figure 6.

Concentration dependence of AGE effects on Ca²⁺ signaling in BAEC (A–C). (D) Correlation between CML content and inhibition of ATP-induced Ca²⁺ increase in BAEC of various AGE samples [(A), 250 mM glucose/90 days; (B), 250 mM G6P/60 days; (C), 50 mM G6P/60 days; (D), 250 mM glucose/60 days; (E), 0.2 mg/ml of 250 mM G6P/60 days; (F), 0.05 mg/ml of 250 mM G6P/60 days]. (E) AGE sample (50 mM G6P/60 days) co-incubated with aminoguanidine (AG, 25 mM) did not alter Ca²⁺ signaling, compared with AGE not containing AG. (F) CML (6 μM) did not alter Ca²⁺ signaling in BAEC. Points and columns represent the mean ± SEM of five to six recordings. *Indicates significant effect of AGE compared to control (P < 0.05, t-test).