nha-oc/NHA2 silencing blocks terminal osteoclast differentiation.
(A) nha-oc/NHA2 silencing inhibits expression of the osteoclast-specific genes nha-oc/NHA2, trap, cathepsin K, mmp-9 and c-src. Expression of β-actin is not affected. nha-oc/NHA2silencing also inhibits osteoclast resorption (bottom panel). Experiments were done in triplicates. * p < 0.05.
(C) Expression of c-Fos and NFATc1, like that of TRAP, is not affected by nha-oc/NHA2 silencing, which strengthen the notion that NHA-oc/NHA2 activity is required for terminal osteoclast differentiation.