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. Author manuscript; available in PMC: 2013 Mar 11.
Published in final edited form as: J Immunol. 2011 Feb 14;186(6):3401–3409. doi: 10.4049/jimmunol.1000328

FIGURE 3.

FIGURE 3

Antitumor effects of IL-12 and 4D5 are dependent on NK cells. A, PBS or the combination of IL-12 and 4D5 was administered to CT-26HER2/neu tumor-bearing mice that had been depleted of NK cells via administration of anti-asialo GM1 (see Materials and Methods for depletion protocol). Mock-treated mice received an isotype-matched control Ab. This treatment resulted in >95% depletion of NK cells from the peripheral blood and spleen, as determined by flow cytometry (data not shown). B, Serum was harvested from each of the mice 24 h after the final dose of IL-12 and 4D5 and analyzed for IFN-γ content by ELISA. *p <0.001 versus all conditions shown. This experiment was repeated twice with similar results.