Table 2. Summary of clinicopathology data and results of PCRs and immunohistochemistry in MCPyV-DNA-positive tumours.

					PCR			Real-time PCR	RT—PCR		Immunohistochemistry
Case	Age	Sex	Clinical stage	Smoking status	LT1	LT3	VP1	MCPyV DNA load (copies per cell: × 10−3)	LT-RNA	VP1-RNA	Tumour	Adjacent tissue
SCC3	73	F	IA	N	+	−	−	0.6	NT		2+	−
SCC15a	78	M	IB	F	+	+	+	26.0	+	−	3+	−
SCC17	74	M	IB	F	−	−	+	0.4	−	−	−	−
SCC21	71	M	IIA	C	−	+	−	NT	NT		2+	1+
SCC22	73	M	IB	N	−	+	−	0.1	−	−	−	−
SCC23	71	M	U	N	−	+	+	0.5	NT		NT
SCC24	69	M	IV	F	−	+	−	NT	NT		2+	−
SCC27	75	F	IA	N	+	+	+	1.5	−	−	1+	1+
SCC32	83	M	IA	N	−	+	−	0.3	NT		−	−
AC7	78	M	IB	F	+	+	−	1.1	NT		2+	−
AC8	59	M	IIIA	F	+	−	+	0.6	NT		1+	1+
AC15	70	F	IIIB	N	−	+	−	0.2	+	−	1+	1+
AC16	74	F	IB	N	+	+	+	0.7	−	−	2+	1+
AC34	73	M	IIIA	F	−	+	−	0.8	NT		1+	1+
AC35	71	F	IIB	F	+	+	+	4.6	NT		3+	−
AC37	67	M	IIIA	F	+	+	+	0.7	−	−	1+	−
AC39	66	F	IIB	F	+	+	+	2.9	+	−	3+	1+
AC43b	81	F	IIB	N	+	+	−	0.5	+	−	3+	1+
LCNEC27	79	M	IIA	F	−	+	−	NT	NT		−	−
PL2	68	M	IIB	F	−	+	+	0.8	−	−	−	−

Abbreviations: AC=adenocarcinoma; C=current smokers; F=female; F=former smokers; LCNEC=large-cell neuroendocrine carcinoma; LT=large T; M=male; MCPyV=Merkel cell polyomavirus; N=never smokers; NT=not tested; PL=pleomorphic carcinoma; RT=reverse transcription; SCC=squamous cell carcinoma; U=unknown; VP=viral protein.

^aMCPyV integration was found at chromosome 5q23.

^bMCPyV integration was found at chromosome 11q25.