Table 2. Synergistic, additive or antagonistic effects on CCID formation by drug combinations.
| Compound(s) | Concentration (μℳ) | CCID formation (% of control)±s.d. | Predicted valuea |
|---|---|---|---|
| A |
20 |
80.73; ±29.63 |
|
| N |
20 |
75.48; ±18.42 |
|
| P |
20 |
83.87; ±10.85 |
|
| I |
20 |
71.87; ±11.57 |
|
| A and I |
20/20 |
55.12; ±18.33
a |
58.02 |
| N and I |
20/20 |
46.43; ±21.89
b |
54.25 |
| P and I |
20/20 |
38.35; ±27.80
b |
60.28 |
| P and A |
20/20 |
77.53; ±34.95
c |
67.71 |
| N and A |
20/20 |
49.93; ±17.43
b |
60.93 |
| I, A, N and P |
20/20/20/20 |
28.59; ±13.91
b |
36.73 |
| N |
40 |
63.67; ±29.16 |
|
| P |
40 |
73.20; ±21.77 |
|
| N and P | 40/40 | 66.15; ±14.11c | 46.60 |
Abbreviations: A=acetohexamide; I=isoxsuprine; N=nifedipin; P=proadifen.
Co-cultivated MCF-7 spheroids and LECs were treated with individual drugs or with the indicated drug combinations for 4 h. Then, CCID formation (% of control) was measured and analysed by Calcusyn software. The CCID data are means of at least 12 determinations; s.d.
a
Predicted value indicating additive effect: (% of dug ‘1' × % of drug ‘2')/100.
b
Synergistic combination effect according to the equation of Chou and Talalay (1984).
c
Antagonistic combination effect according to the equation of Chou and Talalay (1984).
Bold values are the data derived from drug combinations.