Figure 6.

EphA7 overexpression does not alter gross thalamocortical connectivity. (A and A′) The site of DiI injection to label thalamocortical projections was confirmed as a small hole in the MGBv (arrowheads) at the center of relatively darker labeled region. A′ is the grayscale image of nuclear counterstaining with DAPI (blue in A). (B and C′) Anterogradely labeled thalamocortical projections show grossly normal distribution in the layer VI (arrows) and layer IV (arrowheads) of the Actx in EphA7-electroporated brains. C and C′) are higher magnification views of the boxed area in B and B′. B′ and C′ are single channel images of the red channel in B and C. (D) Scatter plot showing the relationship between the densities of EGFP⁺ neurons and DiI-labeled thalamocortical axons in the layer IV of the Actx in EphA7-electroporated brains. No correlation was found between them (Pearson correlation product, R = −0.15, P = 0.08). Scale bars: 500 μm.