Figure 3.

Antigen specific cytokine responses measured from splenocytes and serum antibody response after primary genital infection with C. muridarum. Spleens from different groups of mice were collected at 14 days after primary genital challenge with C. muridarum and levels of IFN-γ, TNF-α and granzyme B produced by splenocytes were measured from supernatants 72 h after in vitro stimulation of the splenocytes with UV-inactivated EBs, unrelated antigen, or medium alone. Results are represented as mean ± SEM for each cytokine per mouse group. ^*Significant (p ≤ 0.05, ANOVA) difference between the indicated groups. Results are representative of two independent experiments. Total Ig from the different groups of mice were measured against UV inactivated EBs in serum collected from infected animals on day 40 after primary genital challenge with C. muridarum. Results are represented as mean ± SEM of reciprocal titers corresponding to 50% maximum binding. ^*Significant (p ≤ 0.05, ANOVA) difference between the indicated groups. Results are representative of two independent experiments.