Figure 5.

Chlamydial clearance following secondary genital infection with C. muridarum. Sixty days following primary genital infection with C. muridarum, mice from each animal group (n = 6) were treated for 4 days with Doxycycline (30 μg/mouse/day) to ensure that the animals cleared Chlamydia. After the Doxycycline treatment, mice were swabbed every third day for 9 days to document chlamydial clearance. All mice were then given Depo-progesterone (day 70), and 5 days later re-challenged intravaginally with 5 × 10⁴ EBs. Mice were swabbed and analyzed for chlamydial IFU recovered every third day for 9 days. Results are represented as mean ± SEM of chlamydial recovery per mouse group at each time point. ^*Significant (p ≤ 0.05, One-Way ANOVA) differences between MHC II^−/− and WT or between MHC II^−/− and TNF-α^−/− mice at the indicated time periods. Results are representative of two independent experiments.