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. Author manuscript; available in PMC: 2013 Mar 11.
Published in final edited form as: J Biol Chem. 2008 Jan 8;283(11):6687–6695. doi: 10.1074/jbc.M705606200

FIGURE 1. JunD inhibits AP-1-mediated transcriptional activation, but not DNA binding, following liver I/R injury.

FIGURE 1

JunD−/− and JunD+/− mice were infected (intravenously) with Ad.AP-1Luc 3 days prior to I/R injury (45 min of partial lobar hepatic ischemia followed by the indicated times of reper-fusion). The 0-h time point represents non-I/R-injured control animals. A, AP-1 transcriptional activity was determined by measuring luciferase activity in liver lysates that were generated at the indicated post-reperfusion time points. Results depict the mean (±S.E.) for n = 4 animals tested at each experimental point. RLU, relative light units. B, nuclear extracts were also generated at each of the reperfusion time points and used in EMSAs to evaluate the DNA binding activity of AP-1. The positions of the AP-1-shifted and free probes are marked by arrows to the left of the gel. The samples on this gel are representative of those for four animals tested at each experimental point. Because of the limited number of lanes on a single gel, the JunD+/− zero time point is not shown but demonstrated no AP-1 shift as shown for JunD−/− mice.