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. Author manuscript; available in PMC: 2013 Mar 11.
Published in final edited form as: J Biol Chem. 2008 Jan 8;283(11):6687–6695. doi: 10.1074/jbc.M705606200

FIGURE 5. JunD modulates NADPH-dependent superoxide production in the liver following I/R injury.

FIGURE 5

JunD−/− and JunD+/− mice were infected (intravenously) with Ad.AP-1Luc and Ad.LacZ or Ad.dnJNK1 viruses 3 days prior to I/R injury (partial lobar ischemia for 45 min followed by the indicated times of reperfusion). The 0-h time point represents non-I/R-injured control animals. At each time point of reperfusion, the liver was harvested and used to generate total endomembranes for an assessment of NADPH-dependent superoxide production using lucigenin chemiluminescence. A, relative NADPH-dependent superoxide production in endomembranes from JunD−/− and JunD+/− mice infected with the indicated adenoviruses. Results depict the mean (±S.E.) for n = 4 animals at each experimental point. B and C, samples assessed in A were used to determine the fraction of DPI-sensitive NADPH-dependent superoxide for JunD−/− (C) and JunD+/− mice (D). In these studies, the endomembrane samples were treated with DPI (10 μm) or vehicle for 30 min prior to the addition of NADPH and measurements of lucigenin chemiluminescence. The fractions of non-DPI-sensitive and DPI-sensitive chemiluminescence are plotted for each sample (n = 4 animals in each group).