Figure 2.

Suboptimal expression of IL-2Rα on Teff (CD8⁺CD44^highCD62L^low), and IL-7Rα/IL-15Rα on Tmem (CD8⁺CD44^highCD62L^high) in allo-Ag sensitized BKO hosts, compared with WT counterparts. At time-intervals after skin grafting, RBC-free peripheral blood cells were stained with Ab against CD8a, CD44, and CD62L plus IL-2Rα, IL-7Rα, or IL-15Rα, and analyzed by four-color flow cytometry. IL-2Rα^highCD62L^low (A), IL-7Rα^highCD62L^high (B), and IL-15Rα^highCD62L^high (C), were gated on CD8⁺CD44^high population, and subtracted from isotype controls. The averages of percentages are shown at serial time-points post B/c skin engraftment (*p<0.01, **p<0.001, n=5/group). The experiments were repeated twice with similar results.